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  5. Analytical Methods

Cluster 02

Analytical Methods for Peptide Characterisation

Orthogonal method coverage: chromatographic purity, molecular identity confirmation, and the contaminant classes that a purity percentage alone never reports.

Articles in this cluster

Technical Guide

How to Evaluate Peptide Purity, Identity, Microbial Quality, and Trace Contaminants

A technical guide to reading research peptide analytical data: RP-HPLC purity, LC-MS identity confirmation, USP <61>/<62>/<71> microbial standards, GC-MS residual solvents, and ICP-OES elemental impurity screening.

September 2, 202614 min read
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Technical Guide

What Are Research Peptides? A Technical Guide to Peptide Identity, Purity & Quality

Research peptides are peptide materials supplied for laboratory research rather than clinical use. This technical guide explains peptide identity, purity, peptide content, impurities, analytical testing, and how to read a lot-specific Certificate of Analysis.

September 8, 202616 min read
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Research

Purity Standards: Why 99% HPLC Doesn't Tell the Whole Story of Quality

HPLC purity is only one dimension of a research compound's true quality. Understanding what a 99% number does — and doesn't — represent is essential for reproducible science.

July 29, 202610 min read
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Research

Acetate vs. TFA Peptide Salts: A Critical Review of Stability and Research Considerations

This review examines how acetate and trifluoroacetate (TFA) salt forms can influence peptide stability, analytical characterization, reconstitution properties, and assay behavior. It also explains why salt form alone cannot predict shelf-life and why peptide-specific stability data, counterion analysis, moisture content, and formulation conditions should guide research decisions.

August 12, 202613 min read
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Peptide Science

Salicylic Acid–Peptide Compatibility: Stability and Formulation Considerations

A technical review of formulation compatibility and peptide stability under acidic conditions: pH-driven degradation pathways, chelation effects on copper peptides, and the RP-HPLC and LC-MS methods used to verify compatibility in vitro.

August 3, 202623 min read
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Technical Guide

How to Read a Peptide COA: Purity Testing, HPLC & Mass Spectrometry

Learn how to evaluate a peptide Certificate of Analysis by checking batch traceability, RP-HPLC purity, mass-spectrometry identity, peptide content, water, counterions, and supporting data.

September 14, 202612 min read
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Analytical Guide

Peptide Purity Explained: How to Read HPLC Purity Results

Learn what a 99% peptide purity result means, how RP-HPLC area purity is calculated, and why purity, identity, and peptide content require separate evidence.

September 18, 202611 min read
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Analytical Guide

Peptide Identity vs Purity: What HPLC and LC-MS Actually Tell You

Understand how HPLC purity, LC-MS identity evidence, and quantitative peptide content answer different analytical questions.

September 28, 202610 min read
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Analytical Guide

Peptide-Related Impurities: Formation, Detection and Interpretation

A researcher's guide to sequence variants, oxidation, stereochemical impurities, co-elution, and the complementary methods used to investigate them.

October 2, 202612 min read
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Topics in preparation

Planned additions to this cluster. Contact our team if you need any of this documentation ahead of publication.

  • RP-HPLC Purity Determination for Peptides

    Gradient design, detection wavelength, resolution and system suitability, and how related substances are integrated and reported.

  • Residual Solvents, Elemental Impurities, and Water Content

    GC headspace residual solvent screening, ICP-OES/ICP-MS elemental impurities, and Karl Fischer or loss-on-drying water content.

  • Microbial Quality and Endotoxin Considerations

    Bioburden enumeration, specified microorganism testing, and how microbial attributes are reported on research-use documentation.

  • Peptide Content vs. Chromatographic Purity

    Why net peptide content, counterion mass, and water content change the actual amount of peptide weighed into an assay.

Other clusters

Peptide FundamentalsCompound ResearchCertificate of Analysis libraryResearch peptide catalog